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Evaluation of the Speed-oligo Mycobacteria assay for the rapid differentiation of frequent Mycobacterium ssp – a multi-centre and multi-country survey

S Hofmann-Thiel, L Turayev, T Alnour, L Drath, M Müllerova, H Hoffmann · Pneumologie · Ausgabe 03/2011

DOI 10.1055/s-0031-1272260

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Autor:innenS Hofmann-Thiel, L Turayev, T Alnour, L Drath, M Müllerova, H Hoffmann
ZeitschriftPneumologie
Ausgabe03/2011
Jahrgang / SeitenNicht angegeben

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A new DNA line probe assay (Speed-oligo Mycobacteria, Vircell) was evaluated for the differentiation of mycobacterial species. It is based on PCR targeting the 16S-23S rRNA internal transcribed spacer region followed by hybridization to specific oligonucleotides by means of a simple dip stick technique. In a first phase of the study the technical validity was evaluated using 166 strains comprising representatives of…

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S Hofmann-Thiel, L Turayev, T Alnour, L Drath, M Müllerova, H Hoffmann (2011) Evaluation of the Speed-oligo Mycobacteria assay for the rapid differentiation of frequent Mycobacterium ssp – a multi-centre and multi-country survey. Pneumologie (03). https://doi.org/10.1055/s-0031-1272260

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