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Establishing short term cultures from human brain tumours and metastases to analyze reduction of cell proliferation by antitumoural drugs

S Grube, J Walter, P Dünisch, D Freitag, R Kalff, C Ewald · Klinische Pädiatrie · Ausgabe 11/2012

DOI 10.1055/s-0032-1320169

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Autor:innenS Grube, J Walter, P Dünisch, D Freitag, R Kalff, C Ewald
ZeitschriftKlinische Pädiatrie
Ausgabe11/2012
Jahrgang / SeitenNicht angegeben

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Abstract-Vorschau

We established a cultivation method for tumour cells derived from operative specimens of human brain tumours of different WHO grades as well as brain metastases. Until now 62 tumour specimens were obtained at the time of craniotomy directly in the OR. These samples were processed to single cell suspensions and plated out. We succeeded in maintaining 65% of these cultures growing over a period of 4–8 weeks. These cul…

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S Grube, J Walter, P Dünisch, D Freitag, R Kalff, C Ewald (2012) Establishing short term cultures from human brain tumours and metastases to analyze reduction of cell proliferation by antitumoural drugs. Klinische Pädiatrie (11). https://doi.org/10.1055/s-0032-1320169

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