CareLit · DOI-Nachweis · Zeitschrift für Gastroenterologie
Preservation of transporter function in 3D primary human hepatocyte cultures allows liver toxicity studies
DOI 10.1055/s-0032-1331998
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Autor:innenA Bachmann, J Martínez Sánchez, X Yan, S Ehnert, G Damm, B Burkhardt, A Nüssler
ZeitschriftZeitschrift für Gastroenterologie
Ausgabe01/2013
Jahrgang / SeitenNicht angegeben
Kernaussage aus der Literatur
Abstract-Vorschau
Introduction: Primary human hepatocytes (hHeps) in 2D culture are the “gold standard” for in vitro toxicity studies. Since these conditions do not reflect tissue in vivo properties, hepatocytes rapidly lose their biotransformation capacity. To mimic in vivo conditions, we developed a 3D culture system maintaining hepatocytes polarity and function. Materials and methods: hHeps were isolated with two-step collagenase…
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Zitierempfehlung
A Bachmann, J Martínez Sánchez, X Yan, S Ehnert, G Damm, B Burkhardt, A Nüssler (2013) Preservation of transporter function in 3D primary human hepatocyte cultures allows liver toxicity studies. Zeitschrift für Gastroenterologie (01). https://doi.org/10.1055/s-0032-1331998
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