CareLit · DOI-Nachweis · Diabetologie und Stoffwechsel
Temperature-dependent effects of glucose and potassium depolarization on granule number and mobility in the submembrane space of insulin-secreting MIN6 cells
DOI 10.1055/s-0034-1375040
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Autor:innenK Schumacher, M Matz, K Baumann, I Rustenbeck
ZeitschriftDiabetologie und Stoffwechsel
Ausgabe05/2014
Jahrgang / SeitenNicht angegeben
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Abstract-Vorschau
Background and aims: TIRF microscopy has changed our view on insulin granule behaviour. Here, we characterized the number and mobility of submembrane granules when insulinotropic stimuli were applied at physiological and sub-physiological temperatures. Methods: Submembrane granules of MIN6 cells were labeled by insulin-EGFP and imaged by TIRF microscopy. The cells were perifused with Krebs-Ringer medium at 37 °C, 32…
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Zitierempfehlung
K Schumacher, M Matz, K Baumann, I Rustenbeck (2014) Temperature-dependent effects of glucose and potassium depolarization on granule number and mobility in the submembrane space of insulin-secreting MIN6 cells. Diabetologie und Stoffwechsel (05). https://doi.org/10.1055/s-0034-1375040
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