Introduction: Vitamin D (VitD) is classically activated via hepatic 25-hydroxylation and renal 1α-hydroxylation, leading to the production of bioactive 1,25(OH)₂D₃. Keratinocytes possess the entire enzymatic machinery for local VitD activation. Although it is known that keratinocytes are able to produce VitD3 from 7-dehydrocholesterol via UVB light exposure and hydroxylated vitamin D metabolites, their role in contributing to an increase in plasma 25(OH)D levels is rather unknown. In addition, there is scarce information on whether basal keratinocytes differ from differentiated keratinocytes in UVB-exposed synthesis of D vitamers.

Objectives: This study investigates UVB-induced VitD metabolism in HaCaT keratinocytes, comparing basal and differentiated keratinocytes, which simulate the basal and upper layers of the epidermis, respectively. The aim is to determine whether keratinocytes can synthesize and secrete 25(OH)D3 following UVB exposure and to identify potential differences between epidermal layers.

Methods: HaCaT keratinocytes were cultured under basal and differentiation conditions and exposed to UVB irradiation for 2 h, 6 h, and 24 h. The expression of key VitD hydroxylases (CYP27A1, CYP27B1) was analyzed via qPCR, and VitD metabolites (VitD₃, 25(OH)D) were quantified using HPLC-MS/MS.

Results: Basal HaCaT cells, mimicking the proliferative" /> Introduction: Vitamin D (VitD) is classically activated via hepatic 25-hydroxylation and renal 1α-hydroxylation, leading to the production of bioactive 1,25(OH)₂D₃. Keratinocytes possess the entire enzymatic machinery for local VitD activation. Although it is known that keratinocytes are able to produce VitD3 from 7-dehydrocholesterol via UVB light exposure and hydroxylated vitamin D metabolites, their role in contributing to an increase in plasma 25(OH)D levels is rather unknown. In addition, there is scarce information on whether basal keratinocytes differ from differentiated keratinocytes in UVB-exposed synthesis of D vitamers.

Objectives: This study investigates UVB-induced VitD metabolism in HaCaT keratinocytes, comparing basal and differentiated keratinocytes, which simulate the basal and upper layers of the epidermis, respectively. The aim is to determine whether keratinocytes can synthesize and secrete 25(OH)D3 following UVB exposure and to identify potential differences between epidermal layers.

Methods: HaCaT keratinocytes were cultured under basal and differentiation conditions and exposed to UVB irradiation for 2 h, 6 h, and 24 h. The expression of key VitD hydroxylases (CYP27A1, CYP27B1) was analyzed via qPCR, and VitD metabolites (VitD₃, 25(OH)D) were quantified using HPLC-MS/MS.

Results: Basal HaCaT cells, mimicking the proliferative" />

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29.05.2025

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