Guideline for testing chemical disinfectants regarding their virucidal activity within the field of human medicine
Rabenau, H.F.; Schwebke, I.; Blümel, J.; Eggers, M.; Glebe, D.; Rapp, I.; Sauerbrei, A.; Steinmann, E.; Steinmann, J.; Willkommen, H.; Wutzler, P. · Bundesgesundheitsblatt - Gesundheitsforschung - Gesundheitsschutz · 2020 · Heft 4 · S. 645 bis 655
Bibliografische Angaben
Zusammenfassung
1 IntroductionThis guideline describes the performance of suspension testing of activity of chemical disinfectants against viruses. These tests must be conducted both without and with the addition of fetal calf serum (FCS) as protein load. A titre reduction of at least four logarithmic steps (4 log10) allows the conclusion that the disinfectant tested has virus-inactivating properties under the tested conditions.Several parameters of the in vitro tests can influence the results (e.g. virus, cells, passage number, cytotoxicity). Furthermore, the titration conditions for the determination of the viral concentratio…
