Surface Motion as a New Loading Concept to Induce Superficial Zone Protein / Lubricin Expression in Tissue Engineered Cartilage

Introduction: Dynamic loading is a valuable tool for in vitro conditioning of tissue engineered cartilage grafts. Surface motion may provide additional stimulation. In the present study the effect of cyclic compression and surface motion on gene expression of chondrocytes seeded onto polymer scaffolds was investigated, with specific emphasis on SZP/lubricin, which is thought to play a key role in maintaining a functional articular surface and joint lubrication. Methods: Cylindrical (8mmx4mm) polyurethane scaffolds were seeded with bovine articular chondrocytes and subjected to cyclic compression and/or surface m…