A novel assay to test multidrug-resistance of mucosa-infiltrating and peripheral blood lymphocytes in patients with inflammatory bowel disease (IBD)
R Schwab, I Peták, B Németh, E Schäfer, Gy Kéri, T Gyökeres, J Hamvas, M Tejeda, Á Pap · Zeitschrift für Gastroenterologie · 2004 · Heft 05
Bibliografische Angaben
Zusammenfassung
Background: Many of current drugs to treat IBD are substrates of ABC-MDR transporters. Until now, no methods were available to screen patients for individual transporter status, although expression of these proteins can be induced by drug therapy and it may change during ongoing treatment. Methods: We have modified a commercial functional assay for detection of MDR-transporters (SOLVO MDQ test). Biopsies from inflamed gut mucosa were dissociated to a single cell suspension. Afterwards, lymphocytes were stained by allophycocyanin (APC) labeled CD45 antibodies. It is necessary, that the emission spectrum of the fl…
