Augmenter of Liver Regeneration Modulates Hepatic Metabolism by Reduction of Cytochrom P450 Activity in Human Hepatocytes in Vitro

Hypothesis: Pathological disorders of the liver were shown to be associated with an impairment of hepatic drug metabolism, particularly that mediated by cytochrome P450s (CYP). Cytokines and growth factors, regulators of liver regeneration, were identified to modulate CYP expression. Recently, a novel liver specific hepatotrophic growth factor ALR, augmenter of liver regeneration, were found to have beneficial effects on liver growth, whereas its action on CYP metabolism is fully unknown. Methods: Primary human hepatocytes in vitro were treated with recombinant human ALR followed by determination of proliferatio…