Study of human amnion cells outgrowth in three-dimensional Collagen I and Fibrin matrices for the treatment of PPROM
G Bilic, H Hall, AG Bittermann, T Burkhardt, P Zammaretti, N Ochsenbein, R Zimmermann · Zeitschrift für Geburtshilfe und Neonatologie · 2005 · Heft 11
Bibliografische Angaben
Zusammenfassung
PPROM occurs in approximately 1% of all pregnancies and 30–40% of preterm deliveries. No satisfactory treatment of PPROM is currently available. Here we studied the human amnion epithelial and mesenchymal cells cultured in three-dimensional (3D) collagen I and fibrin matrices with the objective to develop an implant for application in PPROM. To mimic the architecture of native amnion, amnion mesenchymal cells were embedded in 3D matrices and epithelial cells were placed on top of these matrices. Cell viability, morphology and proteolytic activity of matrix metalloproteinases(MMPs) were investigated. Epithelial a…
