SELDI-TOF-MS Profiling of serum for detection of liver cirrhosis and hepatocellular carcinoma in chronic hepatitis C

The aim of the present study was to establish protein profiles using surface enhanced laser desorption/ionisation time-of-flight mass spectrometry (SELDI-TOF-MS) for identification of liver cirrhosis and hepatocellular carcinoma (HCC) in patients with chronic hepatitis C. Serum samples of 40 patients with F1/F2 fibrosis, 44 patients with F4 fibrosis, 34 patients with HCC were applied to CM 10 cation exchange protein chips and analysed using the SELDI-TOF ProteinChip System (PBS-IIc; Ciphergen Biosystems) after anion-exchange fractionation. Fibrosis stage and HCC had been histologically confirmed in all patients.…