CareLit Fachartikel

Down-regulation of a DNA damage barrier in aging human mammary epithelial cells

T Dörk, B Wieland, B Wieland, M Thren, P Hillemanns, R Hass · Geburtshilfe und Frauenheilkunde · 2008 · Heft 09

Dokument
1010916
CareLit-ID
Jahr
2008
Publikation
PDF
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Metadaten
DOI
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Bibliografische Angaben

Zeitschrift
Geburtshilfe und Frauenheilkunde
Autor:innen
T Dörk, B Wieland, B Wieland, M Thren, P Hillemanns, R Hass
Ausgabe
Heft 09 / 2008
Seiten
–
Erschienen: 2008-09-12 00:00:00
ISSN
0016-5751
DOI
10.1055/s-0028-1089144

Zusammenfassung

We aimed to investigate the capability of human mammary epithelial cells (HMEC) to respond to ionising radiation with the activation of the ATM-Chk2-p53 DNA damage response. HMEC at different passages were exposed to 0.1–6 Gy of ionising radiation, and cellular radiation responses were assessed by flow cytometry and by immunoblot analyses. Early passages of HMEC phosphorylated p53(Ser15), Nbn(Ser343) and Chk2(Thr68) in response to irradiation with 0.1 to 6 Gy in a dose-dependent manner. Inhibitor studies with KU–55933 revealed that these phosphorylation events were strictly dependent on the ataxia-telangiectasia…

Schlagworte

ATM HMEC ionising radiation p53 senescence Geburtshilfe und Frauenheilkunde