Genetic inhibition of telomerase induces alternative lengthening of telomeres (ALT) in genetically defined human esophageal epithelial cells
A Queisser, M Thaler, A von Werder, EM Egenter, S Heeg, N Hirt, H Kunert, S Hauss, H Harada, H Nakagawa, S Li, EH Blackburn, HE Blum, OG Opitz · Zeitschrift für Gastroenterologie · 2008 · Heft 09
Bibliografische Angaben
Zusammenfassung
Introduction: Immortalization is an important step in malignant transformation of human cells depending on telomere maintenance. This is either mediated through activation of the enzyme telomerase containing a template RNA (hTER) and the core protein (hTERT) or through a recombination based alternative mechanism (ALT). Little is known about the regulation of these two mechanisms in a single cell. We investigated, whether genetically defined immortalized and malignant transformed cells can switch from one mechanism to the other using specific genetic telomerase inhibitors. Methods: Immortalized Cyclin-D1 (EPC-D1)…
