GC-MS method for detection of endogenous estrogens in urine samples

Breast cancer is the most common cause of death due to malignancy among women worldwide and the majority of the established risk factors are linked to estrogens as the case maybe the endogenous estrogen metabolism. The identification of specific alterations in endogenous estrogen metabolism as risk factor for breast cancer is a topic of extensive research. For simultaneus quantitation of all estrogens and estrogen metabolites of interest, an aforementioned gas-chromatography mass spectrometry method was optimised. After enzymatic deconjugation of estrogen conjugates using β-glucuronidase, solid-phase extraction…