Polar body diagnosis of 263 unfertilised oocytes: Frequent finding of aneuploidies

Introduction: Aneuploidies of human oocytes are presumed to be a major cause of fertilisation failure in assisted reproductive treatment (ART) cycles. As underlying mechanisms of origin, meiotic nondisjunction of bivalent chromosomes as well as unbalanced predivision of chromatids are discussed [1,2]. The purpose of this study was to evaluate the frequency of aneuploidies in oocytes remaining unfertilised during ART and to identify the underlying mechanism of formation by means of polar body diagnosis (PBD) using the fluorescence-in-situ-hybridisation (FISH) technique. Materials and methods: The study concerned…