The novel IL-10 related cytokine IL-26 is increased in active inflammatory bowel disease and intestinal epithelial cells express the functional IL-26 receptor complex
J Dambacher, F Beigel, K Zitzmann, ST Eichhorst, J Otte, J Seiderer, H Diepolder, CJ Auernhammer, T Ochsenkühn, B Goeke, S Brand · Zeitschrift für Gastroenterologie · 2006 · Heft 10
Bibliografische Angaben
Zusammenfassung
Introduction: Human interleukin-26 (IL-26) signals through a receptor complex consisting of IL-20R1 and IL-10R2. Preliminary studies suggest T lymphocytes as primary source of this cytokine. Aims/Methods: The purpose of this study was to analyze IL-26 receptor expression, signal transduction and specific biological functions of this cytokine system in intestinal epithelial cells (IEC). Methods: Expression studies were performed by RT-PCR and quantitative PCR. Signal transduction was analyzed by Western blot experiments and ELISA. Cell proliferation was measured by MTS assay and Fas induced apoptosis by flow cyto…
