Treatment with the NF-κB inhibitor bortezomib leads to suppression of proregenerative cytokines and significant mortality after liver resection

Introduction: After liver resections or transplantations ischemia/reperfusion injury or inflammatory processes that are mediated through activation of the transcription factor NF-κB lead to additional tissue damage. Thus, suppression of NF-κB activation could be beneficial for patient outcome after these procedures. Methods: To study this question, primary Kupffer cells were isolated from 7 week-old mice and primary hepatocytes were isolated from human liver explants. Cells were cultivated for two days, treated with the NF-KB inhibitor bortezomib and stimulated with TLR-3 and TLR-4 agonist. Expression of cytokin…