Assessment of the Relevance of a CD14 Gene Polymorphism on RNA Polymerase II DNA Binding by Haplotype-Specific Immunoprecipitation (HaploChIP)

Aim: Genetic background may influence susceptibility and progression of various diseases. A polymorphic site within the CD14 gene, rs2569190 / C-159T, was described to be associated with soluble CD14 levels (sCD14) in hepatitis C virus (HCV) infected patients. Both, sCD14 and the membrane-associated form of CD14 (mCD14) are important compounds in mediating endotoxin sensing on myeloid and non-myeloid cells. In vitro experiments suggest the T allele of the SNP to sensitize the host for exogenous or endogenous lipopolysaccharide (LPS) via an enhanced CD14 expression. This study aimed at analysing the in vivo relev…