Glioblastoma centre and periphery: two aspects of the same disease
M Glas, BH Rath, M Simon, R Reinartz, D Trageser, A Leinhaas, R Eisenreich, B Steinfarz, T Pietsch, DA Steindler, J Schramm, O Brüstle, U Herrlinger, B Scheffler · Aktuelle Neurologie · 2009 · Heft 10
Bibliografische Angaben
Zusammenfassung
Objective: One of the hallmark features of glioblastoma (GBM) is the infiltrative nature of tumor cells. Even after complete macroscopic resection, some of the most invasive GBM cells remain in the surrounding brain parenchyma. It was the goal of this study to obtain a functional profile of these cells. Methods: Tissue was derived from experimental biopsies performed at the tumor resection margin after completion of standard neurosurgery. Tumor cells were isolated, expanded under controlled in vitro conditions and profiled (qPCR, FISH, FACS, SNP-genotyping, xenotransplantation) in direct comparison to cells obta…
