The hepatoprotective effect of glycine is mediated through upregulation of proregenerative and downregulation of antiproliferative mediators in the liver

Aims: Glycine pre-treatment has recently been shown to improve the outcome after liver transplantation and ischemia-reperfusion injury. Own data revealed that glycine is also hepatoprotective after subtotal (90%) hepatectomy (SH). However, only little is known about the molecular basis of this effect. Methods: To further elucidate the molecular mechanisms responsible for the hepatoprotective effects of glycine we developed quantitative rt-PCRs and a customized inhouse cDNA gene array system for convenient multi-sample expression profiling for 120 genes of choice known to be involved in liver regeneration, apopto…