Methylation status of the p16 tumor-supressor and DAP-kinase genes in colon biopsies from normal and ulcerative colitis samples

Backgroud and Aims: The normal methylation patterns in tumor tissues are altered in numerous genes. Some of these like p16 and DAP-kinase genes may show a hypermethylation pattern in the CpG islands of their promoter regions in colorectal cancer. The aim of the study was to examine the methylation status of these genes in ulcerative colitis. Methods: DNA was extracted from frozen colon biopsies of 5 healthy patients and from 5 with ulcerative colitis, using High Pure PCR Template Purification Kit. Bisulfite conversion of the samples was perfomed with CpGenome DNA Modification Kit. Methylation-specific PCR was ca…