CFTR gene transfer to human cystic fibrosis pancreatic duct cells using a Sendai virus vector
Z Rakonczay Jr, P Hegyi, M Hasegawa, M Inoue, I Ignáth, EWFW Alton, U Griesenbach, G Óvári, J Vág, G Varga, T Takács, J Lonovics, BE Argent, MA Gray · Zeitschrift für Gastroenterologie · 2007 · Heft 05
Bibliografische Angaben
Zusammenfassung
Aims: Cystic fibrosis (CF) is a fatal inherited disease caused by the absence or dysfunction of cystic fibrosis transmembrane conductance regulator (CFTR), an epithelial cell Cl– channel. About 70% of CF patients have insufficient exocrine pancreatic function due to failure of the pancreatic ducts to secrete a HCO3--rich fluid. Our aim in this study was to investigate the potential of a recombinant Sendai virus (SeV) to introduce normal CFTR into human CF pancreatic duct cells, and to assess the effect of CFTR gene transfer on the key transporters involved in HCO3- transport. Methods and results: Polarised cultu…
