Transgenic overexpression of Abcb11 enhances biliary bile salt outputs but does not affect cholesterol cholelithogenesis in mice

Cholesterol gallstone disease is a complex genetic trait and induced by multiple but as yet unknown genes. A major Lith gene, Lith1 was first identified on chromosome 2 in gallstone-susceptible C57L mice compared with resistant AKR mice. Abcb11 encoding the canalicular bile salt export pump in the hepatocyte, is co-located within the Lith1 QTL region and its hepatic expression is significantly higher in C57L mice than in AKR mice. To investigate whether Abcb11 influences cholesterol gallstone formation, we created an Abcb11 transgenic strain on the AKR genetic background and fed them with a lithogenic diet for 5…