Epigenetic Regulation of Tumor Suppressor AKAP12 in Human Hepatocellular Carcinoma
B Goeppert, P Schmezer, M Breinig, A Warth, MN Vogel, MG Mittelbronn, G Gdynia, K Breuhahn, O Popanda, C Plass, P Schirmacher, MA Kern · Zeitschrift für Gastroenterologie · 2010 · Heft 01
Bibliografische Angaben
Zusammenfassung
Molecular defects leading to hepatocellular carcinoma (HCC), one of the more frequent cancers worldwide, are poorly understood. We have previously shown a downregulation of the tumor suppressor A-kinase Anchoring Protein 12 (AKAP12) in HCC and premalignant lesions. Here, we concentrated on epigenetic gene silencing that may cause the downregulation of AKAP12 in hepatocarcinogenesis. BioCOBRA was used to achieve semi-quantitative DNA methylation data of the AKAP12 gene promoter in a panel of representative specimens (n=39). This analysis revealed a mean DNA methylation of 25.1% for HCC, 2.4% for CL, and 6.6% for…
