Methylation profiling by direct sequencing

Introduction: DNA methylation takes part in the regulation of gene expression, although the exact mechanisms are incompletely understood. Therefore an exact determination of the degree and distribution of methylation is of great importance. Thus far methods used to examine methylation are mostly restricted to single or several CpG sites. Only sequencing methods provide information on the degree and distribution of methylation. Methods: Direct sequencing of bisulfite PCR products is used to determine the distribution and pattern of methylation. The benefit and restrictions of this method is evaluated using calibr…