The non-conjugated chenodeoxycholate induces severe mitochondrial damage and inhibits bicarbonate transport in pancreatic duct cells

Background: We have recently shown that a high contentration (1 mM) of non-conjugated chenodeoxycholate (CDC) had strong inhibitory effects on the activities of acid/base transporters (Na+/H+ exchanger (NHE), Na+/HCO3- cotransporter (NBC), Cl-/HCO3- exchanger (CBE)) of pancreatic ductal epithelial cells (PDEC). Our aim was to characterize the intracellular mechanisms of the inhibitory effects of bile acids. Methods: Intra/interlobular pancreatic ducts were isolated from the pancreas of guinea pigs. Intracellular pH (pHi) and ATP level (ATP)i of PDEC were measured using microfluorometry. To investigate the effect…