Screening of drug metabolizing capacity of the liver using peripheral blood sample. CYP-phenotype, allele and genotype frequencies
L Kóbori, E Sárváry, Z Gerlei, J Fazakas, A Doros, D Görög, I Fehérvári, B Nemes, M Temesvári, P Porrogi, J Paulik, K Monostory · Zeitschrift für Gastroenterologie · 2011 · Heft 05
Bibliografische Angaben
Zusammenfassung
Drug-metabolizing capacity of the liver or transplanted liver graft depends on levels and activities of cytochrome P450 enzymes (CYP). Liver injury increase the morbidity and mortality rate after liver surgery or transplantation. Inter-individual variations in CYP genes result in differences in drug metabolism. Metabolomic and transcriptomic tools were used for CYP-phenotyping, based on the fact that strong correlation exists between CYP enzyme activities in the liver and expression at mRNA level in leucocytes. For CYP-genotyping genomic DNA was extracted from the leukocytes or from the liver tissues of the dono…
