Increased cysteine-protease activity in fecal supernatants from constipated IBS-C patients leads to occludin enzymatic degradation in colonic mucosa
R Róka, A Annaházi, V Bezirard, H Eutamene, L Ferrier, P Ducrotté, O Inczefi, A Rosztóczy, G Ollé, K Gecse, T Molnár, T Piche, V Theodorou, L Bueno, T Wittmann · Zeitschrift für Gastroenterologie · 2011 · Heft 05
Bibliografische Angaben
Zusammenfassung
Background and aims: We have previously demonstrated that (i) fecal cysteine-protease (CP) activity is elevated only in constipated IBS (IBS-C) patients among IBS subtypes and (ii) repeated mucosal exposure to this luminal factor enhances colorectal sensitivity and increases intestinal permeability in mice via the degradation of occludin. We aimed to further explore if IBS-C fecal supernatant is able to degrade the recombinant tight junction protein, occludin in vitro and if occludin levels are decreased in colonic biopsies from IBS-C patients. Methods: Fecal samples and colonic biopsies were collected from heal…
