Establishment and characterisation of primary GBM cell lines

Glioblastoma multiforma still has a very poor prognosis even with today's aggressive combination therapy of resection followed by radio-chemotherapy. This underlines the necessity of new therapeutical strategies. Established glioblastoma cell lines may contain numerous selection artefacts, and they do not represent the large heterogeneity of molecular characteristics in patients. An elegant yet simple solution is the establishment of permanent individual patient tumor cell lines. These represent an invaluable source for detailed molecular, biochemical or functional analyses. Based on a close cooperation and logi…