p53, p63, p73 and the mitochondrial apoptosis signaling pathway in human hepatocellular carcinoma (HCC)

In addition to their functions as nuclear transcription factors p53 and its family members can act in the cytosol and mitochondria to promote apoptosis through transcription-independent mechanisms, including direct protein-protein interactions (PPI) with various members of the Bcl–2 family. Targeting transcription-independent p53 family-mediated cell death may provide a promising strategy for p53-based cancer therapy.To study PPI in a liver cell-based model we adapted LUMIERtechnology (Luminescence-based Mammalian IntERactome mapping, in liver cells. LUMIER is based on the co-purification of two tagged proteins…