Two Photon Microscopy for Clinical Use in Dermatology

Objective: Two Photon Microscopy (TPM) is an imaging method producing high definition images out of the volume of samples, e.g. of human tissue. Up to now the technology and application parameters limit the method to research application. A new approach will enable the TPM to make the step into a method for clinical application. Material and methods: To realize the imaging by TPM a near infrared femtosecond-laser was used to excite endogenous fluorophores in the skin. The advantage of the two-photon-excitation is that the fluorescence is located only in the laser focus. By moving the laser beam through the tissu…