Ethanol and non-oxidative Ethanol metabolites decrease the intracellular ATP level and the bicarbonate secretion in pancreatic ductal epithelial cell line

Introduction: Pancreatic ductal epithelial cells (PDEC) have important roles in the maintenance of pancreatic integrity and bicarbonate secretion. Excessive ethanol consumption is one of the most common causes of acute pancreatitis, but the effects of ethanol metabolites on PDEC are unknown. The aim of this study was to characterize the effects of ethanol and its non-oxidative metabolites on PDEC. Materials and methods: Changes of intracellular ATP level [(ATP)i], pH (pHi) and Ca2+ concentration ([Ca2+]i) of CAPAN-1 cells were measured using microfluorometry in human pancreatic adenocarcinoma cell line (CAPAN-1)…