Improved outcome of FAT mice in a model of acute lung injury in spite of increased spleenic lymphocytes apoptosis
J Ott, M Krebs, C Wolff, A Kiessling, A Buchbinder, J Grabitzki, M Hecker, J Roth, W Seeger, K Mayer · Pneumologie · 2012 · Heft 05
Bibliografische Angaben
Zusammenfassung
ALI and sepsis are still major causes of mortality in intensive care units. After the first hyperinflammatory stage an immunparalyis characterized by apoptosis of spleenic lymphocytes (SL) ensues. Lipid mediators derived from n-6 fatty acids (FA) control inflammation and apoptosis. N-3 FA derived mediators display reduced inflammatory potency. FAT mice are capable of synthesizing n-3 FA from n-6 FA. We induced ALI in wildtype (WT) and FAT mice using 10µg lipopolysaccharide (LPS) intratracheally (i.t.) and isolated lymphocytes from lung (LL) and spleen 0, 4 and 24h later. Using FACS, we differentiated the lymphoc…
