Title. Imaging the dynamics and interactions of granulocytes and antigen-processing cells in allergic airway inflammation by two-photon microscopy

A major characteristic of allergic asthma is the influx of granulocytes and antigen processing cells into the airways. Although it is known that granulocytes can influence antigen processing cells during an immune reaction, an interaction in an acute allergic airway inflammation is unknown. To increase our understanding of the dynamics and interactions of granulocytes and antigen processing cells during airway inflammation, we performed real-time ex vivo imaging of tracheae from mice with an ovalbumin (OVA) induced acute airway inflammation. Tracheal tissue and granulocytes were detected by autofluorescence. Ant…