Characterization of a novel Fibroblast growth factor 10 (Fgf10) knock-in mouse line to target mesenchymal progenitors during embryonic development

Fibroblast growth factor 10 (Fgf10) is a key player for branching morphogenesis in murine embryos. Fgf10-knockout mice suffer from lung and limb agenesis as well as cecal and colonic atresia and are thus not viable. To date, the Mlcv1v-nLacZ-24 transgenic mouse strain (often referred to as Fgf10LacZ), in which a LacZ cassette has been inserted within regulatory sequences 114 kb upstream of the endogenous Fgf10 gene, has been the only strain that allows transient lineage tracing of Fgf10-positive cells. Here, we describe a novel Fgf10Cre-ERT2 knock-in line (or simply Fgf10iCre) in which a Cre-ERT2-IRES-YFP casset…