Genotyping of KIR/KIRL mismatch, FCGR2A, -3A and -3B polymorphisms in patients subject to high-risk neuroblastoma immunotherapy using melting curve analysis

Introduction: Immunotherapy with anti-GD2 monoclonal antibody (mAB) ch14.18 prolongs survival of patients with high-risk neuroblastoma (NB). Since clinical response to some mAbs is associated with immunoglobulin-like receptor (KIR)/KIR-ligand (KIRL) mismatch and Fcγ-receptor (FCGR) polymorphisms, we established a Real-Time-PCR (qPCR) analysis of KIR, FCGR2A (H131R), -3A (V158F) and -3B (NA1/NA2) polymorphisms. Methods and Results: Protocols of qPCR with primers for KIR, FCGR2A, -3A and -3B and subsequent melting curve analysis were established allowing for genotype determination. Importantly, all protocols inclu…