Evaluation of efficacy of antiangiogenic treatment using US-based techniques in a murine model of hepatocellular carcinoma (HCC) under different treatment schedule

Backgroud and aim: An enhanced tumor invasiveness and metastatization during antiangiogenic treatments have been reported due to escape pathways from antiangiogenic-treatments. The aim was to evaluate the tumor response to sorafenib during drug stopping using ultrasound-based imaging techniques in a HCC model. Methods: 5 × 106 Huh7 cells were subcutaneously inoculated in the right flank of nude mice. When tumor-size reached 5 – 10 mm, 14 mice were divided in 3 groups: group-1 was treated with placebo; group-2 was treated until D-5 with sorafenib (62 mg/kg by gavage), then sorafenib was stopped until D-9 and rest…