Hepadnaviral replication in HBV-transgenic mice lacking the surface antigen (HBsAg) is controlled by toll-like receptor 3-induced immune responses
CI Real, R Broering, M Hossbach, J Deckert, K Jahn-Hofmann, L Ickenstein, M John, K Kleinehr, S Driftmann, T Kemper, HP Vornlocher, R Schirmbeck, M Lu, G Gerken, JF Schlaak · Zeitschrift für Gastroenterologie · 2014 · Heft 08
Bibliografische Angaben
Zusammenfassung
Background and aims: It is likely that HBV surface antigen (HBsAg) impairs toll-like receptor (TLR) signaling leading to an attenuation of innate and adaptive immune responses. Here, we aimed to analyze TLR3 signaling in HBV transgenic mice lacking the HBs antigen (1.4 tgHBV-s-mut). Methods: HBxAg-targeting siRNAs or ligand for TLR3 (poly I:C) were injected intravenously into 1.4 tgHBV-s-mut mice and HBV negative littermates. Liver tissue of TLR3-/- and TLR3-/-/1.4 tgHBV-s-mut animals was also investigated. Quantitative RT-PCR, Southern blot, ELISA and western blot were performed to detect levels of immune genes…
