Beneficial Effects on Hepatic Steatosis in a Murine Obesity Model after IL-1 Type Cytokine Inactivation
J Wohlfahrt, A Fettelschoss, T Kündig, H Hermanns, B Müllhaupt, J Schmitt, A Geier · Zeitschrift für Gastroenterologie · 2014 · Heft 09
Bibliografische Angaben
Zusammenfassung
Background: IL-1α/β plays a crucial role in the development of NAFLD, a common cause for chronic liver disease. We want to assess the suitability of cytokine intervention as a therapeutic target for NAFLD and clarify pathophysiological effects of IL-1. Methods: C57BL6-mice were subcutaneously vaccinated with virus-like particles presenting IL-1α/β-antigens, fed a high-fat/high-sucrose diet (HFSD) at w14 and sacrificed at w20. Robust antibody responses were assessed by ELISA, triglyceride levels measured by TG-assay and frozen liver sections stained with H&E/SudanIII to gauge fat accumulation. Expression patterns…
