Genomic high resolution profiling of single CK+/CD45- CTCspurified by flow sorting fromclinical CellSearch samples
R Neves, K Raba, O Schmidt, E Honisch, F Meier-Stiegen, B Behrens, B Möhlendick, T Fehm, H Neubauer, CA Klein, B Polzer, C Sproll, JC Fischer, D Niederacher, N Stoecklein · Geburtshilfe und Frauenheilkunde · 2014 · Heft 09
Bibliografische Angaben
Zusammenfassung
Circulating tumour cells (CTCs) detected by CellSearchTM are important prognostic markers. To learn more about their biology and to use CTCs for clinical applications in sense of a liquid biopsy, we established and tested a workflow for purification of CTCs from CellSearchTM cartridges using a flow sorting approach and that allows their subsequent high-resolution genomic profiling. 34 CellSearchTM pre-processed breast cancer clinical samples were used for single CTCs sorting in a MoFlo sorter. Genome of single CTCs was amplified using an MseI adapter-linker-based PCR. Amplification products were analysed by high…
