DNA methylation markers for the triage of high-risk human papillomavirus infected women
A Hansel, D Steinbach, C Greinke, M Schmitz, J Eiselt, C Scheungraber, M Gajda, H Hoyer, IB Runnebaum, M Dürst · Geburtshilfe und Frauenheilkunde · 2014 · Heft 09
Bibliografische Angaben
Zusammenfassung
Aim: To identify and validate highly discriminating DNA methylation markers for the triage of hrHPV-positive women with or without clinically relevant lesions. Methods: Hypermethylated DNA enriched from cervical cancers was compared with that from cervical scrapes of HPV16-positive cases with no evidence for disease by CpG island microarray hybridization. The most promising marker regions were validated by quantitative methylation-specific PCR (qMSP) using DNA from archived cervical tissues and cervical scrapes. The performance of these markers was then determined in an independent set of 217 hrHPV-positive cerv…
