MicroRNA-9 is a regulator of PDGFRβ expression in pulmonary arterial hypertension
A Weiss, K Gernert, S Schaefer, L Maegel, D Jonigk, A Ghofrani, N Weissmann, F Grimminger, W Seeger, R Schermuly · Pneumologie · 2015 · Heft 07
Bibliografische Angaben
Zusammenfassung
Introduction: In several diseases, proteins are differentially expressed compared to healthy conditions due to microRNAs which serve as translational repressors. Therefore, we aim to define the role of microRNAs in the de-regulation of PDGFRβ expression and their impact on the onset and progression of PAH. Methods: In this project, a variety of techniques commonly used in molecular biology were applied. Western blot analysis and realtime PCR were performed for expression profiling of proteins and mRNAs/microRNAs in cell extracts from lung tissue of mice and humans. The hypoxia-induced mouse model was used to res…
