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Fndc4, a highly identical ortholog of Irisin binds and activates a novel orphan receptor G-protein coupled receptor

A Georgiadi, X Ma, M Bosma, E Graham, O Shilkova, F Mattijssen, AA Khan, JCA Higareda, T Wünsch, M Johansson, S Seaman, BS Croix, O Ritvos, N Nakamura, S Hirose, M Scheideler, S Herzig, PA Böstrom · Diabetologie und Stoffwechsel · 2016 · Heft 04

Dokument
1022700
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Jahr
2016
Publikation
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Bibliografische Angaben

Zeitschrift
Diabetologie und Stoffwechsel
Autor:innen
A Georgiadi, X Ma, M Bosma, E Graham, O Shilkova, F Mattijssen, AA Khan, JCA Higareda, T Wünsch, M Johansson, S Seaman, BS Croix, O Ritvos, N Nakamura, S Hirose, M Scheideler, S Herzig, PA Böstrom
Ausgabe
Heft 04 / 2016
Seiten
–
Erschienen: 2016-04-19 00:00:00
ISSN
1861-9002
DOI
10.1055/s-0036-1580814

Zusammenfassung

Aim: Irisin, encoded by the Fndc5 gene, was identified as an exercise-induced, secreted protein promoting beige cell formation. Here we explore Fndc4, a highly identical ortholog of Fndc5. Fndc4 is proteolytically cleaved, to release an extracellular polypeptide, which we named Irisin2. Methods: In order to investigate the biological effects of Irisin2 we manufactured a stable recombinant Irisin2 and used it in a flow cytometry based assay and immunoprecipatation assays to identify candidate receptors for Irisin2. Results: Recombinant Irisin2 showed superior stability compared to Irisin and greater cellular bind…

Schlagworte

Diabetologie und Stoffwechsel