Epigenetic profiling identifies MIR10A-5 p as a putative tumor suppresor in Ewing sarcoma and rhabdomyosarcoma
DH Martín, F Court, S Rello-Varona, M Sáinz-Jaspeado, R Buj, S Morán, S García-Monclús, J Huertas-Martínez, J Mora, MA Peinado, J Alonso, E de Álava, M Esteller, OM Tirado · Klinische Pädiatrie · 2016 · Heft 05
Bibliografische Angaben
Zusammenfassung
Introduction: Aberrant DNA methylation is thought to be closely related to the development of cancer. Therefore, the identification of specific DNA methylation markers in Ewing sarcoma (ES) and Rhabdomyosarcoma (RMS) would be helpful for understanding the pathogenetic mechanism as well as for developing new therapies. We analyzed the methylome of ES and RMS tumors and cell lines. Methods: Infinium 450k Illumina methylation array. Western blot, qRT-PCR, clonogenic, proliferation and migration assays. iTRAQ proteomic profile. Results: ES and RMS showed hypermethylation in MIR10A-5 p promoter, thus suggesting an in…
