Single Cell Imaging of Human Cologenic Carcinoma Cells by 3-Tesla MRI

Purpose: To detect single human cologenic carcinoma cells (WiDr) loaded with dextran-coated superparamagnetic iron oxide particales (SPIOs) and aminosilane coated SPIOs by high-resolution 3-Tesla MRI and to determine the threshold of iron oxide load for detectability. Method and materials: Incubation of adherent vital monolayer WiDr cells (1×106) with excess concentration of SPIOs (4mg/ml). Application of different incubation times (12 hr? 72 hr). Determination of the average iron load per cell by spectral photometric assessment (605nm wave length). To achieve a 2D-Matrix for MRI scanning WiDr cells were embedde…