CareLit Fachartikel
Diagnosis of malignancy in colon biopsy specimens by mRNA expression profiling
F Sipos, O Galamb, B Molnár, Z Tulassay · Zeitschrift für Gastroenterologie · 2004 · Heft 05
Dokument
1038782
CareLit-ID
Jahr
2004
Publikation
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Metadaten
DOI
ja
zitierfähig
Bibliografische Angaben
Zusammenfassung
Background: The diagnosis of colon biopsy specimen is made by histology where the functional evaluation, genetic analysis of the samples can not be performed. Aim: To develop an mRNA expression analysis by low cost arrays and array technology which can contribute to an enhanced diagnosis. Materials and methods: Total RNA was extracted from healthy and diseased colonic biopsies from 10 patients with adenomatous polyps, 10 with colorectal adenocarcinoma and 10 with inflammatory bowel diseases (5 ulcerative colitis, 5 Crohn's disease). After quantity and quality control, the mRNA fraction was amplified by T7 RNA am…
Schlagworte
Diagnosis
Atlas
Biopsy
Adenomatous Polyps
Adenocarcinoma
Apoptosis
Caspase 8
After
Tumor
Zeitschrift für Gastroenterologie
