Regulatory T cells suppress virus-specific CD8+ T cells in chronically hepatitis C virus infected patients
T Boettler, HC Spangenberg, C Neumann-Haefelin, E Panther, F von Weizsäcker, HE Blum, R Thimme · Zeitschrift für Gastroenterologie · 2004 · Heft 08
Bibliografische Angaben
Zusammenfassung
Introduction: Chronic hepatitis C virus (HCV) infection is characterized by a weak and dysfunctional virus-specific CD8+ T cell response. The mechanisms for this failure are not well understood. In this study, we analyzed the role of CD4+CD25+ regulatory T cells (Treg cells) in the suppression of HCV specific CD8+ T cell responses in chronically HCV infected patients. Methods: PBMCs were obtained from 10 chronically HCV infected patients and 10 healthy individuals. Cell separations (CD4 depletion, CD4+CD25-/+ selection) were performed using magnetic microbeads or dynabeads. CD8+ T cells were tested for HCV pepti…
