Molecular cloning and characterization of Mouse Ntcp promoter: Potent activation by HNF–4a supporting HNF–4 null mice phenotype as well as modest activation by HNF–1a
A Geier, C Gartung, FJ Suchy, CG Dietrich, F Lammert, S Matern, M Ananthanarayanan · Zeitschrift für Gastroenterologie · 2004 · Heft 08
Bibliografische Angaben
Zusammenfassung
Introduction: Sodium taurocholate co-transporting polypeptide (Ntcp) is the major uptake system for conjugated bile acids. Cholestasis leads to downregulation of Ntcp mRNA and protein involving a decrease in nuclear HNF–1 levels in rats. Whether similar mechanisms operate in the regulation of mouse Ntcp gene is unknown. Aim: To clone and functionally characterize mouse Ntcp promoter. Methods: A 129/SV mouse lambda genomic library was screened with a partial mouse Ntcp cDNA and a positive hybridizing clone was isolated. Mouse Ntcp promoter sequence was obtained by DNA sequencing of this clone (Genbank AF190697).…
