Vectorial Transport of CCK–8 in Double-Transfected MDCKII Cells Expressing OATP1B3 and MRP2 (ABCC2)

Cholecystokinin–8 (CCK–8, Sincalide), a small isoform of the gastrointestinal peptide hormone cholecystokinin, is specifically taken up into human hepatocytes by the organic anion transporter OATP1B3, also known as OATP8. It has been demonstrated that CCK–8 is efficiently excreted into rat bile, however, the molecular basis of this transport of CCK–8 across the canalicular membrane remained unknown. Double-transfected MDCKII cells, expressing recombinant human OATP1B3 in the basolateral membrane together with the multidrug resistance protein 2 (MRP2, ABCC2) in the apical membrane represent a valuable model syste…