Unaltered cell cycle regulation after infection with high-capacity but not first-generation adenoviral vectors

Rationale: Adenovirus vectors are widely used in experimental and clinical gene transfer studies to the lung. First-generation (E1/E3 deleted) vectors are well documented to induce severe cell cycle dysregulation in target cells. For high-capacity vectors (all viral genes deleted) information about their influence on cell cycle regulation in the target cell are still missing. Methods: We investigated the influence of high-capacitiy Ad vectors on cell cycle regulation by comparing two high-capacity vectors with different expression cassettes (GFP and human α1-antitrypsin [hAAT]) with a first-generation vector (hA…